Fig. 1: catTFRE proteomics analysis revealed SEL-induced nuclear accumulation of TC molecule Lipin1 in MM cells. | Acta Pharmacologica Sinica

Fig. 1: catTFRE proteomics analysis revealed SEL-induced nuclear accumulation of TC molecule Lipin1 in MM cells.

From: Lipin1-dependent transcriptional inactivation of SREBPs contributes to selinexor sensitivity in multiple myeloma

Fig. 1

a Schematic illustration of the experimental procedure for catTFRE proteomics detection. NCI-H929 cells were treated with either DMSO (n = 3) or 500 nM SEL (n = 3) for 12 h followed by nuclear protein extraction, catTFRE pull-down, mass spectrometry detection and bioinformatics analysis. b The bar plot indicated the number of TFs and TCs detected in each sample. c The volcano plot illustrates the significantly altered TFs/TCs in SEL-treatment group compared to DMSO-treatment group. d Pathway enrichment analysis of upregulated and downregulated TFs/TCs in MM nucleus following SEL treatment. e, f Molecular network of upregulated (e) or downregulated (f) TF/TCs, along with the biological processes in which they are involved. g Protein-protein interaction analysis revealed that Lipin1 interacts with SREBP1 and SREBP2.

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