Fig. 5: Lipin1 contributes to the sensitivity of myeloma cells to SEL in vitro. | Acta Pharmacologica Sinica

Fig. 5: Lipin1 contributes to the sensitivity of myeloma cells to SEL in vitro.

From: Lipin1-dependent transcriptional inactivation of SREBPs contributes to selinexor sensitivity in multiple myeloma

Fig. 5

a MM cells were transduced with lentiviral vectors carrying LPIN1-targeting shRNA (shLPIN1#1, shLPIN1#2) or scrambled control shRNA (shNC). Cell proliferation was assessed using the CCK-8 method. b Cell viability of the indicated MM cells treated with increasing doses of SEL was evaluated using the CCK-8 method. c Cell apoptosis rates in the control and LPIN1-knockdown MM cells treated with 100 nM SEL for 48 h were analyzed using Annexin V/7-AAD dual staining and flow cytometry. d Cell cycle distribution in the control and LPIN1-knockdown MM cells treated with 100 nM SEL for 48 h was analyzed using PI staining and flow cytometry. Data are shown as mean ± SD; The statistical significance for the above data is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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