Fig. 1: Four hit compounds were identified from a natural product library, and their antiviral activity against LCMV infection was assessed. | Acta Pharmacologica Sinica

Fig. 1: Four hit compounds were identified from a natural product library, and their antiviral activity against LCMV infection was assessed.

From: Salvianolic acid A from Salvia miltiorrhiza identified as a cap-dependent endonuclease inhibitor for pathogenic arenaviruses

Fig. 1

a Flowchart of the screening process for cap-dependent endonuclease (CEN) inhibitors from a natural product library, based on both in vitro anti-CEN assays and cellular antiviral assays. b Inhibition of the enzymatic activity of the lymphocytic choriomeningitis virus (LCMV) CEN was detected using our established high-throughput screening system; 150 compounds at 50 µM inhibited LCMV CEN, with over 40% selected for cellular analysis. c The inhibition of viral infection was detected using real-time quantitative PCR; 23 compounds at 50 µM inhibited LCMV infection, with over 40% selected from the initial 150. d Chemical structures of the four hits: salvianolic acid A (SAA), chebulinic acid, tannic acid, and punicalagin. e, f Antiviral effects and cytotoxicity of SAA, chebulinic acid, tannic acid, and punicalagin on LCMV were determined using BHK-21 cells (e) and confirmed using A549 cells (f); EC50s and CC50s, noted along with the curves, were calculated from at least three independent biological replicates.

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