Fig. 4: ZBED6 overexpression prevents PMCFs activation induced by TGF-β in vitro. | Acta Pharmacologica Sinica

Fig. 4: ZBED6 overexpression prevents PMCFs activation induced by TGF-β in vitro.

From: Zinc finger BED-type containing 6 (ZBED6) ameliorates cardiac fibrosis by inhibiting Piezo1 transcription and YAP nuclear translocation

Fig. 4: ZBED6 overexpression prevents PMCFs activation induced by TGF-β in vitro.

a, b ZBED6 overexpression plasmid transfection efficiency was verified by qRT-PCR and Western blot. n = 5–6. *P < 0.05, **P < 0.01 vs. NC. c Cell viability measured by CCK8 in each group. n = 6. **P < 0.01 vs. NC; ##P < 0.01 vs. TGF-β + NC. d, e The proliferation of PMCFs was detected by EdU staining. n = 4. **P < 0.01 vs. NC; ##P < 0.01 vs. TGF-β + NC f, g The migration ratio in each group. Scale bar = 100 μm. n = 4. **P < 0.01 vs. NC; ##P < 0.01 vs. TGF-β + NC. h, i Fluorescence intensity of α-SMA in PMCFs. Scale bar = 20 μm. n = 4. **P < 0.01 vs. NC; ##P < 0.01 vs. TGF-β + NC. j–m The fibrotic genes mRNA expression levels. n = 5. **P < 0.01 vs. NC; ##P < 0.01 vs. TGF-β + NC.

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