Fig. 2: Functional status of total CD8 T cells and leukemia-reactive CD8 T cells in bone marrow vs. peripheral blood of AML. | Blood Cancer Journal

Fig. 2: Functional status of total CD8 T cells and leukemia-reactive CD8 T cells in bone marrow vs. peripheral blood of AML.

From: Bone marrow CD8 T cells express high frequency of PD-1 and exhibit reduced anti-leukemia response in newly diagnosed AML patients

Fig. 2

a, b PBMCs and BBMCs collected from AML patients at initial diagnosis (n = 10) were stimulated in vitro with anti-CD3 and anti-CD28 before intracellular staining with IFN-γ and TNF-α. Flow cytometry analysis of the expression of IFN-γ (a) and TNF-α (b) are shown. Left panels, representative flow data; right panels, summary plots. c, d Expression of Ki67 (c) and Granzyme B (d) in CD8 T cells from bone marrow and peripheral blood of AML patients (n = 22) was assessed by flow cytometry. Representative flow data (left) and summary plot (right) are shown. e CD8 T cells purified from PBMCs or BMMCs were co-cultured with T2 cells (used as antigen presenting cells) pulsed with WT1 or SV40 peptide (used as negative control) for 6 days. After the co-culture, flow cytometry analysis of the intracellular expression of IFN-γ and TNF-α in CD8 T cells was performed (n = 4). Representative flow data (left) and statistic summary plot (right) are shown. f PD-1 expression on leukemia-reactive CD8 T cells (gated on IFN-γ+ cells, n = 4). Representative flow data (top) and statistic plot (bottom) are shown. P values were obtained by the paired t test

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