Fig. 2: FANCD2 foci formation in a FANCL heterozygous cell line model.
From: Rare variants in Fanconi anemia genes are enriched in acute myeloid leukemia
a Immunofluorescent images captured at 63 × magnification for three representative MCF10A CRISPR-FANCL clones; WT, heterozygous (Het-1), and bi-allelic. Cells were treated with mitomycin C (MMC) and probed with DAPI (blue) or antibodies for γH2AX (green), and FANCD2 (red). b Percentage of γH2AX positive cells with no detectable FANCD2 foci in MCF10A CRISPR-FANCL clones. Black: WT clone, gray: heterozygous clones (Het-1, Het-2, Het-3); white: bi-allelic clone. For statistical comparison, One-way ANOVA with Tukey multiple comparison was performed. *P < 0.05, ***P < 0.001, ****P < 0.0001. c Percentage of γH2AX positive cells with FANCD2 foci ≥ 3 in the 5 MCF10A CRISPR-FANCL clones. Black: WT clone, gray: heterozygous clones (Het-1, Het-2, Het-3); white: bi-allelic clone. For statistical comparison, One-way ANOVA with Tukey multiple comparison was performed. *P < 0.05, *** P < 0.001, **** P < 0.0001
