Fig. 4: Combination of huCD26mAb plus novel agents induced significant lysis of CD26+ MM cell lines by ADCC.
CD26+ MM cell lines; KMS18, KMS26, and KMS28, co-cultured with OCs were pretreated with dexamethasone (Dexa, 25 nM), bortezomib (BTZ, 4 nM), and lenalidomide (Lena, 0.5 μM) for 24 h. Then, calcein-AM-labeled CD26+ target MM cell lines were co-cultured with NK effector cells at the E/T ratio of 20 in the presence of huCD26mAb (10 μg/ml) or iso IgG1. The data represent the mean ± SE of triplicate wells (*p < 0.05). b CD26+ MM.1R co-cultured with OCs was pretreated with Dexa (25 nM), BTZ (3 nM), or Lena (0.5 μM). Next, huCD26mAb-triggered ADCC lysis against CD26+ MM.1R in the presence of NK effector cells was assayed by calcein-AM release assay. The data represent the mean ± SE of triplicate wells (*p < 0.05, **p < 0.01). c NK effector cells were pretreated with Lena (0.5 μM) for 24 h followed by huCD26mAb-induced ADCC against CD26+ MM.1R cells cultured with OCs. The data represent the mean ± SE of triplicate wells (*p < 0.05, **p < 0.01)
