Fig. 2: Representative images illustrating the two main patterns of MYC amplification.
From: High level MYC amplification in B-cell lymphomas: is it a marker of aggressive disease?
LEFT: Single Interphase nucleus demonstrating MYC amplification as a “Cloud-like” distribution or innumerable dispersed 5′ MYC signals throughout the interphase nucleus using the 5′ (Red)/3′(Green) break-apart MYC probe. The Chromosome 8 Ideograms demonstrate the location of the intact break-apart MYC probe at 8q24.1. The MYC amplification corresponds to the individual small fragments of red genomic material representing either Double-minutes or Episomal amplification of the 5′ MYC (Red) gene region. RIGHT: Single Interphase nucleus demonstrating MYC amplification as a Homogeneous Staining Region (HSR) or innumerable overlapping MYC gene signals confined to one or more specific locations in the interphase nucleus using the MYC (red) and IGH (green) D-FISH probe set. A single normal interphase nucleus is also present for comparison. The Chromosome 8 Ideograms illustrate the location of the MYC locus at 8q24.1 with a red probe and an example HSR on 8q. The G-Banded Chromosomes 1 from this particular case demonstrate one normal chromosome 1 and two copies of an abnormal chromosome 1 with an HSR on 1p (arrows) corresponding to the MYC amplification identified in the interphase nucleus.
