Fig. 5: Effects of del(11q) and/or BIRC3 mutations in CLL cell lines proliferation and clonal evolution. | Blood Cancer Journal

Fig. 5: Effects of del(11q) and/or BIRC3 mutations in CLL cell lines proliferation and clonal evolution.

From: Biological significance of monoallelic and biallelic BIRC3 loss in del(11q) chronic lymphocytic leukemia progression

Fig. 5

A Analysis of del(11q) and/or BIRC3 mutations on proliferation of HG3 cells after 72 h. MTT absorbance values are normalized with the HG3WT clones. Data are summarized as the mean ± SD. B Viability analysis of HG3WT, HG3-del(11q), and HG3-del(11q) BIRC3MUT cells treated with DMSO or 1 μM NIK SMI1. Cell viability was assessed by MTT assay after 48 h and data is represented as the absolute absorbance value for each independent clone. C HG3-del(11q) RFP-tagged and HG3-del(11q) BIRC3MUT GFP-tagged cells (left panel), HG3WT RFP-tagged and HG3 BIRC3MUT GFP-tagged cells (right panel), were mixed at a ratio 1:1 and left in culture for the indicated days. Clonal evolution was assessed at the indicated time points by flow cytometry. Bars represent mean ± SD.

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