Fig. 2: Phenotypic characterization of γδT-cell subsets.
PBMCs were stained with antibodies reacting to CD3, TCR γδT, CD45RA, and CD27. Upon the analysis on gated CD3 + TCR γδT + cells, the four γδT subsets were identified as (A) naïve, B TCM, C TEM, and D TEMRA. The cell number of each γδT subset in PB were calculated based on the total lymphocyte count. Significant differences were found among γδT subsets isolated from healthy controls and from CML patients at different disease states. Data comparison was performed by the Student’s t test; if data distribution was not normally distributed, nonparametric Mann-Whitney U test was used. Median values were indicated by the short horizontal red bars. Statistical significance was defined as *p < 0.05, **p < 0.01, and ***p < 0.001. ns, not significant.
