Fig. 3: Expansion and activation of γδT and its subsets when co-cultured with CML cells treated with BCR-ABL inhibitors. | Blood Cancer Journal

Fig. 3: Expansion and activation of γδT and its subsets when co-cultured with CML cells treated with BCR-ABL inhibitors.

From: Activated naïve γδ T cells accelerate deep molecular response to BCR-ABL inhibitors in patients with chronic myeloid leukemia

Fig. 3: Expansion and activation of γδT and its subsets when co-cultured with CML cells treated with BCR-ABL inhibitors.

A Expression of isolated γδT cells was determined by CFSE-labeling. CFSE-labeled γδT cells were co-cultured with BCR-ABL-knockdown KCL22 cells (black bar) or untreated KCL22 cells (open bar). Simvastatin-pretreated, zoledronate-pretreated, or BCR-ABL siRNA-pretreated KCL22 cells were used as the targets for γδT cells at a ratio of 1:1. Zoledronate (Zometa, 5 μM) but not simvastatin (100 nM) rendered KCL22 cell proliferation. B Expression of TNF-α, IFN-γ, and perforin release of isolated γδT cells from six healthy donors was determined by ELISA after co-culture with BCR-ABL siRNA-, TKI-, simvastatin-treated, or untreated K562 cells for 24 h. Treatment with IPP (0.5 μM) was used as a control to activate γδT cells. The ex-vivo killing assays were performed using isolated (C) γδT cells and (D) naïve γδT cells isolated from healthy donors. The targets could be untreated KU812 cells, or KU812 pretreated with BCR-ABL-specific siRNA or zoledronate. The incubation time for target-γδT cells and for target-naïve subset was 4 h and 24 h, respectively. One-way ANOVA was used for comparison between multiple groups, and paired t test was used to compare selected groups. Statistical significances between these groups are marked with asterisk. Data were presented as mean values ± SD. Data comparison was performed by the paired t test. Statistical significance was defined by *p < 0.05, **p < 0.01, and ***p < 0.001.

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