Fig. 3: The role of BCL-2 family members in BH3 mimetic–induced apoptosis. | Blood Cancer Journal

Fig. 3: The role of BCL-2 family members in BH3 mimetic–induced apoptosis.

From: Combined inhibition of BCL-2 and MCL-1 overcomes BAX deficiency-mediated resistance of TP53-mutant acute myeloid leukemia to individual BH3 mimetics

Fig. 3

A TP53-WT and -mutant (R175H and R248Q) Molm13 cells were treated with VEN, AMG176 (AMG), or both at the indicated concentrations for 24 h. Protein levels were determined by Western blot analysis. B Schematic of apoptosis induction requiring BAX and BAK activation, which is activated by BIM and blocked by BCL-2 and MCL-1. C, D TP53-WT, -KO, or -mutant Molm13 cells (C) and vector control or BAX-KD Molm13 cells (D) were treated with VEN, AMG176, or both at the indicated concentrations for 24 h. BAX and BAK activation and cytochrome C release were measured by flow cytometry. h hour.

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