Fig. 4: Near-haploid KBM7 cells show elevated expression of RAD51 and RAD51B in post-replication phases and unique genetic dependency on RAD51B compared to diploidized cells.

a Expression patterns of RAD51 and RAD51B throughout the cell cycle in diploid (left panels) and haploid (right panels) KBM7 cells. Arrows highlight cells with peak expression in each cell type for each gene. b Inferred cell cycle pseudo-time trajectory for RAD51 and RAD51B, with estimated 95% confidence interval shown as shaded regions. c Schematic for genome-wide CRISPR-Cas9 gene essentiality screen in haploid and diploid KBM7 cells. d Gene set enrichment analysis (GSEA) of genes essential to haploid but not diploid KBM7 cells. Shown are normalized enrichment scores. All categories show significant enrichment (Kolmogorov–Smirnov test p-values < 0.01 with false discovery rate <0.25). e Volcano plots of relative enrichment/depletion scores of genes (horizontal axis) and the associated significance levels of the scores (vertical axis). RAD51B is highlighted in green. f Venn-diagram highlighting RAD51B and other genes as uniquely important to near-haploid KBM7 cells by comparing essential genes across a panel of cell lines [44], hematologic cell lines, and haploid KBM7 cells (this study and Wang et al. 2015 study [38]). Genes shown were pre-filtered for those showing higher cell cycle stage-specific expression in near-haploid KBM7 cells as identified by our single-cell analyses. g 1-D density distribution plots (darker gray denotes higher relative density of values) of the log2-fold change of individual sgRNAs. Log2-fold change values of sgRNAs targeting the 6 essential genes specific to near-haploid cells as shown in (f) are highlighted with red bars.