Fig. 1: Summary of cytogenetic alterations and TP53 mutations.

A Frequencies of autosomal monosomies (percentages represent frequencies in patients with available data on karyotype). The most frequent autosomies involved chromosomes 7, 17 and 18. B Co-occurrence matrix of autosomal monosomies. Counts indicate frequencies of co-occurrence. The most frequently co-occurring monosomies with loss of 17 were monosomies 7 and 16, followed by monosomies of chromosomes 5, 6 and 12. C Ideogram of global losses (orange) and gains (green) across the genome. Recurrent losses are enriched in chromosomes 5, 7 and 17. Furthermore, we observed additional recurrent losses on 12p, besides 16q, 18p, and 18q. Chromosomal gains were prevalent, particularly on chromosomes 8 (trisomy 8), as well as on chromosomes 1, 9, 11, 21, and 22. Clean karyotypes were batched parsed in CytoGPS to create .json files with loss, gain & fusion information. These files were examined using the RCytoGPS package for R, treating them as a binary matrix, to create the ideogram [36]. D Summary of TP53 mutations in the cohort. Somatic variants in TP53 visualized using lollipop plot generated via the ProteinPaint web-based application [37]. Majority comprised hot-spot DNA-binding domain missense mutations. Chromosomal position coordinates were culled from the IARC database of TP53 mutations. A few complex mutations seen in a few cases are not depicted and numbers may not match up with that depicted in the results section.