Fig. 2
Ca2+-dependent contractions in osteocytes are mediated by smooth muscle myosin. a Western blots for smooth muscle myosin proteins in NIH3T3 fibroblast cells, osteoblast cell line MC3T3-E1, and osteocyte cell line MLO-Y4. Immunofluorescence in MLO-Y4 cells shows co-localization of smooth muscle myosin with the F-actin network. b Representative Ezz trace for MLCK inhibition by ML-7, with a significantly delayed contraction compared to ATP control (see Fig. 1d). c The time before a decrease in Ezz strain after ATP induction was increased from (13.3 ± 6.4) s in control cells (n = 6) to (41.8 ± 24.2) s in ML-7 treated cells (n = 8). Arrowheads indicate initiation of contractile events.*P < 0.05. Error bars are standard deviations.
