Fig. 3

Osteocytes contract micropillar substrates in response to Ca²⁺ induction. a Representative MLO-Y4 cell stained with Fluo-8 AM and the underlying micropillars coated with rhodamine fibronectin. Intracellular Ca²⁺ and pillars are shown before and after the addition of ionomycin. b Time course of Fluo-8 AM intensity and (c) maximum pixel displacement following the addition of ATP of pillars under the cell periphery and static pillars away from the cell (n = 3 cells). d Time course of Fluo-8 AM intensity and (e) maximum pixel displacement following the addition of ionomycin of pillars under the cell periphery and static pillars away from the cell (n = 3 cells). *P < 0.05. Error bars are standard deviations.