Fig. 5
Omentin-1 downregulates the pro-osteoclastic abilities of TNF-α-activated macrophages. a TRAP staining of RAW264.7 cells cultured in RANKL-containing medium supplemented with CM from macrophages treated with PBS (30 min + 24 h), TNF-α (10 ng·mL-1; 30 min), or TNF-α (10 ng·mL-1; 30 min) + omentin-1 (300 μg·mL-1; 24 h). Scale bar: 50 μm. b Quantitative analysis of the number of TRAP+ cells in a. n = 3 per group. c–h The expression levels of osteoclastogenesis-related genes including Trap, Mmp9, Ctsk, ATP6V0d2, Oscar, and NFATc1 were assessed by qRT-PCR analyses. n = 3 per group. All the results are representative of three independent experiments. Data are shown as the mean ± s.d. *P < 0.05 compared with the PBS group (cells treated with CM from inactivated macrophages), #P < 0.05 compared with the TNF-α group (cells treated with CM from TNF-α-activated macrophages).
