Fig. 6
Omentin-1 attenuates inflammatory responses induced by magnesium silicate. a–c Immunohistochemical staining of IL-1β, IL-6, and TNF-α in bone marrow and on bone surface in mice receiving different treatments. Scale bar: 50 μm. d–f Quantitative analyses of the mean intensity of the positively stained areas in a–c. n = 5–7 per group. g Immunohistochemical staining for CD86 (a marker for pro-inflammatory M1 macrophages). Scale bar: 50 μm. h Quantitative analysis of the numbers of CD86+ cells in g. n = 3 per group. i Immunohistochemical staining for CD206 (a marker for anti-inflammatory M2 macrophages). Scale bar: 50 μm. j Quantitative analysis of the numbers of CD206+ cells in i. n = 3 per group. k The serum concentration of IL-1α in different treatment groups, as analyzed by ELISA. n = 5–9 per group. Data are shown as the mean ± s.d. *P < 0.05 compared with the control group, #P < 0.05 compared with the magnesium silicate + Ad-Gal (Mg + Ad-Gal) group.
