Fig. 5
Anti-LTA mAb characterization. Experimental anti-LTA antibody (SAC55) was analyzed for binding characteristics by several methods against species-matched isotype control IgG (R347). a koff, kon, and KD were assessed by Octet for SAC55 against LTA. b Selective binding of SAC55 to Gram-positive bacteria (S. aureus and S. epidermidis) but not Gram-negative bacteria (E. coli and P. aeruginosa) is assessed, as well as an absence of binding by the control antibody R347. c Flow cytometry of in vitro grown S. aureus reveals selective binding, with the distinction between groups improved by addition of human sera. d Flow cytometry of S. aureus harvested from mouse blood shows selective binding of SAC55 to Gram-positive bacteria, following in vivo passage.
