Fig. 9 | Bone Research

Fig. 9

From: Ciliary parathyroid hormone signaling activates transforming growth factor-β to maintain intervertebral disc homeostasis during aging

Fig. 9The alternative text for this image may have been generated using AI.

Primary cilia regulates PTH signaling in NP cells for disc anabolic activity. a 3D PPCT and immunostaining images showing that PTH effect on IVDs diminished in IFT88−/− mice. PPCT Scale bar, 500 μm. Safranin-O Scale bars, 100 μm. b Quantitative analysis of IVD volume and (c) IVD histological scores of (a). d Western blot analysis of pCREB in NP cells isolated from IFT88−/− or IFT88+/+ mice injected with iPTH or vehicle with or without shear stress. e Immunostaining for pSmad2/3 (brown), CCN2 and ACAN (red) with DAPI (blue) in 2-month IFT88−/− or IFT88+/+ mice treated with PTH or vehicle. pSmad2/3 and CCN2 Scale bar, 50 μm; ACAN Scale bar, 100 μm. f, g and h Quantitative analysis of percentages of pSmad2/3 positive cells in NP area and CCN2 and ACAN-positive area of total IVD area. *P < 0.05. n = 8, per group. Statistical significance was determined by one-way ANOVA and Student's t-test. All data are reported as the mean ± s.d.

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