Fig. 4
From: NOTUM inhibition increases endocortical bone formation and bone strength
NOTUM inhibition increases mineralization in osteoblast cell cultures. a Effect of human and mouse NOTUM conditioned media (CM) on osteoblast mineralization (alizarin red staining) in MC3T3-E1 cells (100% human NOTUM CM = 1.2 μg·mL−1; 100% mouse NOTUM CM = 0.4 μg·mL−1) (N = 3 cell cultures per data point). b, c Inhibition of mineralization caused by NOTUM (5% human NOTUM CM) was dose-dependently blocked by addition of NOTUM inhibitors LP-922056 or LP-914822 to the culture medium. Both studies lasted 21 days with NOTUM CM present throughout the study. Mineralizing medium = medium without addition of NOTUM CM. Values are means ± SEM of six cell cultures per data point
