Fig. 6
Administration of anti-Cxcl12 antibody attenuated subchondral bone remodeling and articular cartilage degeneration in ACLT mice. a, b, d, e Representative immunostaining and quantitative analysis of Cxcl12 in Osterix+ preosteoblasts in the subchondral bone and metaphysis in ΔTSC1, ΔRaptor mice, and their littermates (Ctrl). Boxed area is magnified on the corner. Scale bar, 100 μm. AC, articular cartilage; SCB, subchondral bone; GP, growth plate; BM, bone marrow. c Representative Safranin O-Fast green, H&E staining, 3D reconstructed μ-CT images and immunohistochemical staining of OCN, type X collagen, and MMP-13 in ΔTSC1 mice administered with anti-Cxcl12 antibody or normal IgG for 6 weeks. Scale bar, 100 μm (Safranin O, H&E staining, OCN, type X collagen, and MMP-13), 1 mm (μ-CT). f OARSI scores based on the histological analysis. g, h Quantitative analysis of type X collagen and MMP-13 in articular cartilage. i, j Quantitative analysis of bone mass in subchondral bone: bone volume/total volume (BV/TV) and bone mineral density (BMD). k Quantitative analysis of OCN+ osteoblasts in tibial subchondral bone. Data are shown as mean±s.d. and analyzed by Student’s t test. n ≥ 6, **P < 0.01
