Fig. 7 | Bone Research

Fig. 7

From: Collagen type II suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1−SMAD1 interaction

Fig. 7

COL2A1 signaling was associated with OA cartilage degeneration and pathological changes of the subchondral bone. Both injured cartilage (mostly from the rim of the ulcer, comprising ~3–4 mm of the surrounding tissue) and paired macroscopically unaffected cartilage were collected from ten patients with knee OA. a, b Safranin O/Fast green staining, HE staining, and the Mankin score were evaluated. c The expression levels of COL2A1, COL10A1, RUNX2, and ID1 were detected using qPCR in both injured cartilage and paired unaffected cartilage. d IHC staining of COL2A1, COL10A1, RUNX2, ID1, p-SMAD1S463/465, and p-SMAD1S206 was performed, and a semiquantitative analysis of IHC images was conducted. Data in (c) are presented as the mean ± SD (n = 10). *P < 0.05. OA, osteoarthritis; IHC, immunohistochemistry

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