Fig. 7

Mechanical suppression of breast cancer cell invasion and signaling to osteoclasts requires SUN1 and SUN2. a Quantification of cells (normalized to total area) invading through trans-well membrane under control (Con, no LIV) or twice-daily LIV (LIV) conditions. Cells were transfected with control siRNA oligos (siCon) or with siRNAs targeting both SUN1 and SUN2 (siSUN1/2). Data are representative of four biological replicates. Representative images are shown in Supplementary Fig. 6. b Quantification of the number of osteoclasts following exposure of RAW 264.7 cells to conditioned media from MDA-MB-231 cells that received LIV twice-daily for 3 days and transfected with control siRNA sequences (siCon) or siRNAs targeting SUN1 and SUN2 (siSUN1/2) prior to LIV. Data were compiled from ten biological replicates and representative images are shown in Supplementary Fig. 8. c Osteoclast resorption, as measured by the area (%) of hydroxyapatite resorbed by differentiated RAW 264.7 cells on Osteoassay plates following the addition of conditioned media from MDA-MB-231 conditioned media. MDA-MB-231 cells were transfected with siRNA sequences targeting SUN1 and SUN2 (siSUN1/2), as mentioned above, and exposed to twice-daily LIV, or non-LIV control conditions (Con). Data compiled from six biological replicates and measured using ImageJ. Two-way ANOVA P-values: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1