Fig. 6
From: A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone
Effect of Smurf2 on BMP/Smad signaling. a, b Ubiquitination assay of Smad1 and Smad5 ubiquitination by Smurf2. c Western blot analysis with the indicated antibodies to evaluate expression levels in rhBMP2-stimulated BMSCs. d Quantitative real-time PCR analysis of BMSCs cultured in rhBMP2-supplemented osteogenic medium containing DMSO (Vehicle) or dorsomorphin (DM, 4 μmol·L−1). e, f ALP staining of BMSCs cultured in rhBMP2-supplemented osteogenic medium containing DMSO (Vehicle) or dorsomorphin (DM, 4 μmol·L−1) (scale bar = 2 mm). g Model of the BMP/Smad pathway in WT and Smurf2−/− mice. (n = 3 mice per group; *P < 0.05 by one-way ANOVA followed by the Bonferroni test)
