Fig. 1
From: Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling
Isolation and characterization of ABs from BMMs, pOCs, and mOCs. a Graphical illustration of the procedure for AB isolation. b Images of TRAP and IF staining of the cytoskeleton of BMMs, pOCs, and mOCs. Cell nuclei stained with DAPI are shown in blue, and vinculin is shown in red. The scale bars represent 200 μm. c The proportions of TRAP-positive cells and multinucleated TRAP-positive cells were quantified in each well (96-well plate), n = 5. d Heat map showing the expression profile of RANKL-dependent specific osteoclastogenic genes from 0 h (BMMs) to 96 h (mOCs). e Representative images of ABs stained with Annexin-V/FITC. The bar represents 3 μm. f FSC/SSC analysis and Annexin-V/PI analysis of viable cells, apoptotic cells, and ABs. The data in the figures are presented as the average ± SD values. Statistically significant differences between the treatment and control groups are indicated as * (P < 0.05) or ** (P < 0.01)
