Fig. 5
From: Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling
pOC-ABs promote angiogenesis by delivering PDGF-BB to recipient EPCs. a IHC of CD31 in mice 2 weeks after engrafting of AB-DBM. The red arrows indicate new CD31-positive vessels formed in the DRA. The bars represent 1 mm (upper) and 100 μm (lower). b Semiquantitative analysis showing the IHC score for CD31 in mice grafted with AB-DBM, n = 8. c Representative flow cytometric analysis with the percentages of CD31hiEmcnhi cells among total cells digested from the DRA, n = 5. d Immunostaining analysis of CD31 and Emcn with quantitative analysis of the relative immunostaining intensities in the DRA. The bar represents 40 μm, n = 5. e PDGF-BB expression in different ABs, as determined by western blot analysis. As shown, PDGF-BB was included in the proteomic signature of pOC-ABs. f PDGF-BB expression in pOC-ABs isolated from pOCs of TRAP-cre;Pdgfbf/f mice, as determined by western blot analysis. g IHC and semiquantitative analysis of CD31 expression in mice grafted with DBM preincubated with TRAP-cre;Pdgfbf/f pOC-ABs or Pdgfbf/f pOC-ABs. The bar represents 1 mm in the overview and 100 μm in the center region, n = 8. h Representative flow cytometric analysis with the percentages of CD31hiEmcnhi cells among total cells digested from the DRA, n = 5. i Representative images of immunostaining of CD31 and Emcn with quantification of the relative CD31 and Emcn immunostaining intensities in the DRA. The bar represents 20 μm, n = 5. The data in the figures are presented as the average ± SD values. Statistically significant differences between the treatment and control groups are indicated as * (P < 0.05) or ** (P < 0.01)
