Fig. 1
Loss of mTORC1 function in pre-OBs results in a lean phenotype. a Temporal change in body weight (n ≥ 15/genotype). b %Fat normalized to total body weight. c Body weight-adjusted gonadal and inguinal white adipose tissue (gWAT and iWAT) and interscapular brown adipose tissue (iBAT) mass (9 weeks of age; n = 10/group). d Representative H&E section of gWAT and iWAT, scale bar = 100 µm. e, f Size distribution of gWAT and iWAT adipocytes calculated from H&E stained sections using Image J (n = 5 sections/mouse, n = 3–6/genotype). g, h Gene expression levels of adipogenesis, lipogenesis, and lipolysis genes, normalized to Actb, in gWAT and iWAT (n = 3–5/genotype). i Serum triglyceride levels (n = 8–10/genotype). j Serum-free fatty acid levels (n = 8–10/genotype). k Total energy expenditure, normalized to lean mass, over 48 h and l per light/dark cycle. m Average respiratory quotient (RQ) over 48 h and n per light/dark cycle. Data are expressed as mean ± SEM from n = 7/group, unless indicated otherwise. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey’s post hoc test. k, m Shaded regions represent dark cycle
