Fig. 2
Wound healing and migration assay. a Randomly selected images of the gap (500 μm) at 0, 4, 8, 12, and 24 h after treatment with or without LIPUS stimulation. Scale bars, 200 μm. b Changes in the cell-covered area over time. LIPUS stimulation significantly increased the migration and proliferation of MC3T3-E1 cells (n = 3, **P < 0.01, Student’s t-test). LIPUS stimulation also increased the migration and proliferation of shRNA-Piezo1 cells (n = 3, *P < 0.05, Student’s t-test), but the difference was not as obvious as it was in MC3T3-E1 cells. The cell-covered area of MC3T3-E1 cells was significantly higher than that of shRNA-Piezo1 cells at 4, 8, and 12 h after LIPUS stimulation (n = 3, **P < 0.01, Student’s t-test)
