Fig. 1
From: Peptidomimetic inhibitor of L-plastin reduces osteoclastic bone resorption in aging female mice
Osteoclasts were stained for actin with rhodamine-phalloidin, and confocal images of osteoclasts are shown (a–d). An arrowhead points to a small sealing ring in (d). Scale Bar-25 µm. e and f Dentine resorption assay in vitro: Osteoclasts plated on dentine matrix were incubated with scrambled (e) or inhibitory (f) LPL peptide in the presence of TNF-α for 10–14 h. Resorption pits were photographed under a 40X objective of phase-contrast microscopy (magnification is ×400). Resorbed areas of approximately ~25–30 pits were quantified from three slices per treatment/experiment (n = 3) and averaged over two different experiments. Statistical analysis of the pit area was performed by Student’s test and provided at the bottom. ***P < 0.001 vs. scrambled peptide transduced osteoclasts (mean ± SEM)
