Fig. 1

Differential effects of MLO-A5 osteocyte-derived conditioned medium (A5 CM) with and without fluid flow (FF) treatment at 1 Pa on TMD cells. CN control and A5 A5 CM. The single and double asterisks indicate P < 0.05 and P < 0.01, respectively. a Representative images of A5 and Y4 cells and the expression of Sclerostin (Scl) and DMP1. b Inhibition of 2-dimensional cellular migration in a scratch assay by A5 CM. c Stimulation of cell proliferation by A5 CM in an EdU assay. d Compaction of TMD tumor spheroids by A5 CM. e Downregulation of Vimentin (Vim), MMP9, Snail, and Slug and upregulation of E-cadherin (E-cad) and p-Akt by A5 CM. f Promotion of cellular migration in a scratch assay by A5 FFCM. g Reduction in cellular proliferation by A5 FFCM. h Expansion of TMD tumor spheroids by A5 FFCM. i Upregulation of Vimentin, MMP9, Snail, and Slug and downregulation of E-cadherin and p-Akt by A5 FFCM