Fig. 6

Ablation of SLIT3 in hematopoietic cells exhibits a normal bone phenotype and skeletal vasculature. a Schema showing the protocol for BM transplantation. Representative micro-CT images (b), femoral BV/TV, cortical bone thickness, and bone morphology of the distal femur trabecular bone (c) and representative confocal images of DAPI, CD31, and EMCN fluorescence in femur sections (d) isolated from bone marrow chimeras created using 5-week-old Slit3^+/+ and Slit3^−/− mouse donors and WT hosts (n = 6). GP growth plate. Values represent the mean ± s.e.m., n.s. (not statistically significant) by unpaired two-tailed Student’s t test. Scale bars, 500 μm