Fig. 5
From: Aging and menopause reprogram osteoclast precursors for aggressive bone resorption
Comparison of the gene expression level of the TM7SF4 gene, encoding DC-STAMP, with (a) the donor age, or (b) the number of years since menopause (n = 48). Comparison of the gene expression level of the CTSK gene, encoding cathepsin K, with (c) the donor age, or (d) the number of years since menopause (n = 49). Comparison of the percentage of mature cathepsin K (of the total cathepsin K for each donor) within each OC culture, as determined by Western blotting with (e) the donor age (n = 46) and (f) the number of years since menopause (n = 46). (g) Comparison of the relative amount of mature cathepsin K (normalized to the β-actin level) between pre- and postmenopausal women as determined by Western blotting (n = 46). In a, c, and e, green dots indicate premenopausal donors, while black dots indicate postmenopausal donors. Statistical correlation analyses were performed using either Spearman’s rank correlation (rs) or Pearson’s correlation (r2). Comparisons between two groups were performed using the Mann–Whitney test. One data point in a was excluded using the ROUT outlier test (Q = 2%). Each dot represents the results obtained from OCs generated from an individual donor
