Fig. 5 | Bone Research

Fig. 5

From: An enhanced chemopreventive effect of methyl donor S-adenosylmethionine in combination with 25-hydroxyvitamin D in blocking mammary tumor growth and metastasis

Fig. 5

Functional validation of the genes identified by RNA-Seq. a Pathway analysis of the up- and downregulated DEGs from the SAM + 25(OH)D combination-treated group was done by using the KEGG and Reactome databases. Top five up- and downregulated pathways are shown as bar graphs. b GSEA analyses further showed the enrichment of the genes from ‘interferon alpha/beta signaling’ and ‘HIF-1 signaling pathway’. c RNA obtained from the control and treated PyMT-R221A cells was subjected to qPCR to validate the expression of selected genes from the ‘interferon alpha/beta signaling’ and ‘HIF-1 signaling' pathways. Results are shown as mean ± SEM of samples obtained from at least three different experiments per group. Significant differences are represented by asterisks

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