Fig. 1
Mice with Nsd1 knockout in mesenchymal progenitors showed impaired cartilage development. a mRNA levels of H3K36 methyltransferases and chondrocyte differentiation marker genes were determined by qRT-PCR in micromasses at different differentiation time points. The values are presented as the means ± SEMs, n = 4. *P < 0.05, **P < 0.01, ns means not significant. The inset shows Alcian blue staining results of micromasses cultured for 1, 4, and 7 days with chondroprogenitor cells. Scale bar = 2 mm. b SO staining results of E11.5 limb buds. Scale bar = 100 μm. c Whole-mount in situ hybridization (WISH) results for Col2 in E12.5 embryos (top) and sections of forelimbs (bottom). The dashed purple lines show the digits already present. Scale bar (top) = 500 μm, scale bar (bottom) = 200 μm. d SO staining results of E13.5 (first line), E14.5 (second line), E15.5 (third line), and E16.5 (fourth line) femur sections from WT and Nsd1f/f;Prx1-Cre mice. The dashed black lines show the borders between hypertrophic chondrocytes and the primary ossification center. Scale bar = 200 μm. SO staining of P7 (e) and P14 (f) hindlimb sections from WT and Nsd1f/f;Prx1-Cre mice. Scale bar (top) = 200 μm. Scale bar (bottom) = 500 μm. g Whole-mount in situ hybridization (WISH) results for Col2 in E12.5 embryos (top) and sections of forelimbs (bottom). The dashed purple lines show the digits already present. Scale bar (top) = 500 μm, scale bar (bottom) = 200 μm. h SO staining results of E13.5 (top) and E15.5 (bottom) femur sections from WT, Nsd1f/f;Col2-Cre mice. The dashed black lines show the borders between hypertrophic chondrocytes and the primary ossification center. Scale bar = 200 μm. SO staining of P7 (i) and P14 (j) hindlimb sections from WT and Nsd1f/f;Col2-Cre mice. Scale bar (top) = 200 μm, Scale bar (bottom) = 500 μm
