Table 2 Conditional deletion of Piezo1 in experimental mice

Global deletion

⁶³

Embryos died at E9.5 lacking modeling of vasculature

Impaired endothelial cell alignment in response to shear stress; failing to remodel arteries

Endothelial cells

Cadherin5-CreERT2¹⁸¹

Inhibited Ca²⁺ influx induced by Yoda1; more sensitive to α-adrenergic agonists

Cadherin5-CreERT2¹³⁷

Impaired physical performance and lower body weight after sustained activity

Piezo1 transduced fluid flow signal to constriction of mesenteric arteries, which are responsible for total peripheral resistance

iEC-Cre¹³²

Increased lung vascular permeability by high-volume mechanical ventilation

Piezo1 targeted calcium-dependent cysteine protease calpain to maintain homeostasis of the endothelial barrier

iEC-Cre¹³³

Increased lung microvessel pressure; impaired regulation of lung endothelial barrier

Piezo1 regulated lung vascular permeability by targeting endothelial VE-cadherin

Tie2-Cre or Lyve1-Cre¹³⁴

Reduced amount of lymphatic valves

Tie2-CreERT2¹³⁸

Upregulated arterial blood pressure

Failed to produce NO or vasodilate because of insensitivity to flow stimulation

Adult endothelial cells

SCL-CreERT¹³⁶

Impaired angiogenesis; inhibited endothelial sprouting and lumen formation after subjected to wall shear stress

Piezo1 increased intracellular Ca²⁺ and activated MT1-MMP pathway

Lymphatic endothelial cells

Prox1-CreERT2¹³⁵

Inhibited formation and maintenance of lymphatic valves and lymphatic vessels

Piezo1 transduced the signal of OSS to control lymphatic valves and lymphatic vessels

Smooth muscle cells

sm22-Cre¹⁴

Reduced activity of ion channels in caudal artery myocytes induced by stretch; reduced arterial diameter, wall thickness, and cross-sectional area treated with Angiotensin II

Piezo1 sensed flow and pressure to regulate structural remodeling of arteries

Adipocytes

Adiponectin-Cre¹⁴¹

Increased insulin resistance; decreased pgWAT weight and increased pro-inflammatory and lipolysis genes after HFD fed; hepatic steatosis with increased fatty acid synthesis genes

Piezo1 participated in TLR4-mediated inflammation

Adiponectin-CreERT2¹⁴⁰

Impaired adipocyte differentiation, causing inflammation and insulin insensitivity

Piezo1 promoted the secretion of adipogenic FGF1 to facilitate adipocyte precursor differentiation

Myeloid cells

LysM-Cre¹⁴³

Ameliorated pulmonary inflammation

Piezo1 exhibited proinflammatory effects by activating AP-1c and EDN1 and stabilizing HIF1α

Acinar cells

Ptf1a-CreER¹⁴²

Ameliorated pancreatitis, including reduced edema, neutrophil infiltration, hemorrhage, and tissue necrosis

Piezo1 activated cytoplasmic calcium signals that are toxic to pancreatic acinar cells, resulting in cellular necrosis and pancreatitis

Nodose and petrosal sensory ganglia neurons

Phox2b-Cre Piezo1/2 dKO¹⁵⁶

Attenuated baroreflex and activity of aortic depressor nerve

DRG neurons

shRNA KD¹⁵⁹

Inhibited Ca²⁺ influx induced by Yoda1

The mechanism is still unclear whether targeting Ca²⁺ -induced phospholipase C δ, β, or other signaling pathways