Fig. 2
From: IκB-ζ signaling promotes chondrocyte inflammatory phenotype, senescence, and erosive joint pathology
Chondrocytes support osteoclastogenesis under inflammatory conditions. a Primary IKK2f/f chondrocytes were isolated and infected with adenoviral-GFP or adenoviral-cre to delete IKK2. Bone marrow macrophages were then added to the culture. Permissive levels of Rankl (5 ng·mL−1) was added ± IL-1β (10 ng·mL−1) for 3 days. Cultures were fixed and stained for TRAP positive osteoclasts (red arrows). Macrophages alone cultures are displayed as control. b Number of large, multi nucleated cells (MNC) in macrophage-chondrocyte co-culture were quantified by counting. (WT + IL-1β vs IKK2−/− + IL-1β ***P = 0.000 2, n = 4 wells from representative experiment). Bars represent mean ± S.D. c mRNA was isolated from co-cultures of chondrocytes and macrophages ± IL-1β. Gene expression analysis was performed for TRAP (**P = 0.001 7, n = 4). Bars represent mean ± S.D.
