Table 2 Summary of studies in which skeletal repair has been assayed with reduced Hh signaling
From: On the horizon: Hedgehog signaling to heal broken bones
Reference | Injury type | Hh LOF | Assay | Results | Conclusions |
|---|---|---|---|---|---|
Mouse 4-mm femoral autograft | Rosa-CreER;Smof/f | MicroCT Histology | • Reduced bone and callus formation • More undifferentiated cells and fibrotic tissue • Reduced proliferation | • Hh signaling drives expansion of the early repair callus | |
Mouse tibial fracture | Esr1-Cre;Smof/f | Radiography Histology Histomorphometry | • Smaller callus, 28 days post-fracture | • Hh signaling modulates repair quality by enhancing osteoblast differentiation but is not required | |
Col2-Cre;Smof/f | • No change, 28 days post-fracture | • Hh signaling is dispensable for chondrocytes | |||
Col1-Cre;Smof/f | • Smaller callus, decreased bone tissue, 28 days post-fracture | • Hh signaling promotes osteoblast activity and matrix production | |||
Mouse fibular fracture | Col2a1-Cre;Ihhf/f | X-ray MicroCT Immunohistochemistry | • Reduced cartilage callus but no change in bone formation or overall fracture repair | • Hh signaling affects cartilage but is not required for fracture repair | |
Zebrafish mandibular resection | Ihha mutant | Histology Alizarin red/Alcian blue RNA-ISH MicroCT | • Reduced cartilage callus and bone formation, poor bone quality | • Hh signaling is required for cartilage induction during regeneration | |
Mouse Rib resection | Sox9-CreER;Smof/f | Histology Alizarin red/Alcian blue RNA-ISH | • Reduced cartilage callus and bone formation | • Hh signaling is required for cartilage induction during regeneration |
