Fig. 6: Sialidase-mediated reductions in osteoclast formation and bone remodeling were abrogated in Siglec15ΔLysM mice. | Bone Research

Fig. 6: Sialidase-mediated reductions in osteoclast formation and bone remodeling were abrogated in Siglec15ΔLysM mice.

From: Sialylation of TLR2 initiates osteoclast fusion

Fig. 6

a Representative μCT cross-sectional femur images of Siglec15fl/fl (WT) and Siglec15ΔLysM mice intrafemorally injected with vehicle (VEH) or sialidase. The bar represents 4 mm. b Quantitative μCT analysis of trabecular number (Tb.N), trabecular thickness (Tb.Th), bone volume/tissue volume (BV/TV), and trabecular connectivity (Tb. Con); n = 5. c TRAP staining of distal femur sections from Siglec15fl/fl (WT) mice intrafemorally injected with vehicle or sialidase and quantification of the multinuclear osteoclast number, n = 3. d Immunostaining of α2,3 SA and TLR2 in distal femur sections from Siglec15fl/fl (WT) mice intrafemorally injected with vehicle or sialidase and colocalization analysis of α2,3 with TLR2. e Body weight and femur length of Siglec15fl/fl (WT) and Siglec15ΔLysM mice intrafemorally injected with vehicle or sialidase, n = 5. f Schematic diagram showing the self/nonself-recognition mediated by siglec15 binding with sialylated TLR2 before further cell fusion. Data represent the mean ± SD, and statistically significant differences are indicated as *P < 0.05; **P < 0.01; ***P < 0.001

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