Fig. 1

ZsGreen labels SCs and FAPs in skeletal muscles of the Pax7^ZsG and Prrx1^ZsG mice, respectively. a Schematic representation of tamoxifen-inducible Cre-dependent ZsGreen reporter induction. b Pax7^ZsG (n = 4/group) and Prrx1^ZsG (n = 3/group) mice were injected with CDTX in the hamstring muscle of the right hind limb (RHL) and PBS in the hamstring muscle of the left hind limb (LHL). Muscle cells were isolated 14 days after injection and subsequently stained for surface cell markers. c After gating on forward/side scatters and FVS700 dead cell exclusion, ZsGreen-high cells were gated from Lin⁻ CD45⁻ nonhematopoietic population in both (d) Pax7^ZsG and (e) Prrx1^ZsG mice and further subgrouped into CD31⁺Sca1⁺(EC), CD31⁻Sca1⁻CD34⁺(SCs), CD31⁻Sca1⁺CD34⁺ (FAPs), and Sca1⁻CD34⁻ populations. The color indicates the expression level of (i) integrin α7 and (ii) PDGFRα. Yellow-orange: high expression. Green: low expression. f Frequencies of ZsGreen⁺ cells in Sca1⁺CD31⁺ ECs, CD31⁻ Sca1⁻ CD34⁺ ITGA7⁺ PDGFRα⁻ SCs and CD31⁻ Sca1⁺ CD34⁺ ITGA7⁻ PDGFRα⁺ FAP populations from muscles of the Pax7^ZsG and Prrx1^ZsG mice following the gating strategy in Fig. S1. Each dot represents a separate mouse, and bars are the mean ± SD. The results of 8 mice per group were from 3 independent experiments performed over a period of 2 years with different flow cytometers. Significance was analyzed by one-way ANOVA with Tukey’s multiple comparison test