Fig. 4 | Bone Research

Fig. 4

From: Generation of functional oligopeptides that promote osteogenesis based on unsupervised deep learning of protein IDRs

Fig. 4The alternative text for this image may have been generated using AI.

AIB5P enhances bone formation in vivo. Six mice in each group were administered AIB5P or vehicle (100 μg·kg−1, every 3 days, intravenous, total 1.5 months). Femurs were collected for further experiments. a High-magnification calcein and xylenol orange double labeling in femurs. Bar = 100 μm. The distance between the two fluorescent lines shows the amount of new bone deposition. b Quantification of the mineral apposition rate (MAR) and the bone formation rate per bone surface (BFR/BS) value. n = 6. c, d von Kossa staining and relative quantification of femur sections. Bar, 200 μm. Samples from six mice were used for statistics. e, f Representative anti-DMP1 IHC staining images and semiquantification. Samples from six mice were used for statistics. g Representative micro-CT of mouse femurs. Upper, longitudinal transverse sections at midshaft scans. Bar, 1 mm. Middle, trabecular bone underneath the growth plate. Bar, 500 μm. Lower, cortical bone. Bar, 500 μm. h Quantification analysis of micro-CT. Bone material density (BMD), bone volume per tissue volume (BV/TV), trabecular bone numbers (Tb.N), trabecular bone space (Tb.Sp) and trabecular bone thickness (Tb.Th) of trabecular bone compared between the vehicle control and AIB5P groups. BV/TV of the cortical bone was also compared. n = 6

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