Fig. 3
Enhanced metaphyseal bone formation in 9-month-old Adq-GsαR201C mice. a, b Representative histological pictures from Sirius red-stained sections and histomorphometric analysis of trabecular bone of lumbar vertebrae (a) and femora (b). BV/TV: bone volume per tissue volume. c Representative images from H&E-stained sections showing osteoblasts (arrowhead) and quantitative histomorphometry of osteoblast parameters. N.Ob/BS: number of osteoblasts per bone surface. Ob.S/BS: osteoblast surface per bone surface. d Representative pictures of calcein labeling in trabecular bone of female mice and dynamic bone formation parameters. MS/BS: mineralizing surface per bone surface. MAR Mineral apposition rate. BFR/BS Bone formation rate. e Relative gene expression of Alpl, Sp7 and Bglap in femora from female mice. f TRAP histochemistry highlighting cells of the osteoclastic lineage (arrow) and quantitative histomorphometry of osteoclast parameters. N.Oc/BS: number of osteoclasts per bone surface. Oc.S/BS: osteoclast surface per bone surface. g Relative gene expression of Rankl, Opg, Rankl/Opg ratio and Rank in femora from female mice. F Females. M Males. Data are presented as the mean ± SEM. Statistical analysis was performed using Student t-test; *P < 0.05, **P < 0.01, ****P < 0.000 1. The exact P-value was reported on male column bars in a and b
