Fig. 6
Diaphyseal intramedullary bone formation is reproduced in Adq-GsαR201C male mice by 17β-estradiol (E2) treatment. a Experimental scheme of E2 treatment started at 5 months of age. b Radiographs of dissected tibiae and femora at the end of E2 treatment. Arrowheads indicate the increased density in the diaphyseal region of E2-treated bone segments from Adq-GsαR201C mice. c Representative longitudinal and transverse micro-CT images of Veh- and E2-treated mice, showing the diaphyseal intramedullary bone in E2-treated Adq-GsαR201C male mice. Transversal images were taken 2 mm above the tibia-fibular junction. d Sirius red stained sections of the tibial midshafts showing intramedullary bone in Adq-GsαR201C male mice after 6 weeks of E2 treatment. e Representative confocal images showing GFP-labeled intramedullary bone in E2-treated Adq-GsαR201C male mice. No bone is observed in Veh-treated mice. f Cluster of perivascular GFP-labeled stromal cells (asterisk) preceding the appearance of bone with GFP-labeled osteoblasts (arrowhead) and osteocytes (hollow arrowhead) in the marrow cavity of E2-treated Adq-GsαR201C mice. g Schematic representation of GFP-labeled medullary bone formation by Adq-GsαR201C marrow perivascular/stromal cells. mb Medullary bone, bm Bone marrow, cb Cortical bone, bv Blood vessel
