Fig. 6 | Bone Research

Fig. 6

From: Runx1 is a key regulator of articular cartilage homeostasis by orchestrating YAP, TGFβ, and Wnt signaling in articular cartilage formation and osteoarthritis

Fig. 6The alternative text for this image may have been generated using AI.

Runx1 overexpression in chondrocytes promoted Yap expression in vitro, and local Runx1 overexpression by AAV protected against OA in an ACLT mouse model in vivo. a Images under a fluorescence microscope. pMXs-GFP (GFP) and Runx1 overexpression (Runx1-OE) by pMXs-3xFlag-Runx1 retrovirus transfection in ATDC5 cells that were induced to chondrocytes for 14 days. Bar: 100 μm. b Alcian blue staining in ATDC5 cells that were induced to differentiate into chondrocytes for 14 days. Bar: 100 μm. c Western blot of induced cells. d Schematic display of ACLT surgery-induced OA and subsequent AAV-Runx1/AAV-YFP treatment workflow. e Images under a fluorescence microscope for YFP expression in treated mouse knee joints. Bar: 50 μm. f Radiography results showed that AAV-Runx1 prevented ACLT-mediated OA. The arrow shows osteophytes and worn articular cartilage. g Knee joint safranin O (SO) staining of AAV-treated ACLT-mediated osteoarthritic knees. The blue line shows the space of the knee joint. Bar: 50 μm. h OARSI score of (g). The results are presented as the mean ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001

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