Table 1 Comparison of different reporter mice based on Cre/loxP system
Reporter mice | Visualization | Features | References |
|---|---|---|---|
R26R-LacZ | X-gal staining | Capable of whole-mount visualization; staining cannot be performed in live-tissue | |
R26R-EYFP R26R-tdTomato | Fluorescence | Capable of live-tissue visualization and co-staining with other markers | |
Double-fluorescent mT/mG | Fluorescence | Cells express tdTomato (mT) and GFP (mG) before and after Cre-mediated excision respectively; the non-recombined cells function as an internal control; suitable for mosaic analysis | |
R26R-Confetti/Brainbow2.1 | Fluorescence; confocal microscope recommended | Usually bred with CreER and the recombination efficiency is affected by tamoxifen; stochastic recombination; capable of multicolor visualization (green, yellow, red, blue), distinguishment of adjacent cells, and clonogenic assay | |
Transgenes-GFP/mCherry/DsRed | Fluorescence | Expression is directly driven by transgenes and is Cre/loxP system-independent, widely used in combination with Cre/loxP system |
