Fig. 6

The activation of PKM2 mitigates colitis-induced osteoclastic bone loss. a Representative μCT images of the femurs of mice treated with DSS or the control with or without TEPP46. Bone volume per tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular spacing (Tb.Sp), bone mineral density (BMD), and cortical thickness (Ct.Th) are shown (n = 4–6 mice per group). Scale bars represent 0.5 mm. b Representative TRAP staining images of tibias in (a). TRAP⁺ osteoclast size (OC size) and number (OC.N/BS) are shown. The scale bar represents 200 μm. GP Growth plate, Metap. Metaphysis. c Representative dynamic histomorphometry of tibias in Panel (a). The mineral apposition rate (MAR) and bone formation rate (BFR) are shown. The scale bar represents 20 μm. d Serum levels of TNF-α, IL-6, and IL-17AF from the mice in (a) were measured by ELISA. Each dot represents the result of a single mouse. Each dot in OC size represents the result of a single osteoclast. The data are presented as the means ± S.D