Fig. 5 | Bone Research

Fig. 5

From: Neutralization of excessive levels of active TGF-β1 reduces MSC recruitment and differentiation to mitigate peritendinous adhesion

Fig. 5

Immunofluorescence staining (a) and the number (b) of Nestin+ (green) cells per PAF tissue area (mm2) on postoperative Days 1, 3, 7, 14, 21, and 28. Immunofluorescence staining (c) and the number (d) of α-SMA+ (red) cells per PAF tissue area (mm2) at 1, 3, 7, 14, 21, and 28 days after tendon injury. White arrowheads indicate the space between the tendon and surrounding tissues. Yellow arrowheads indicate the space occupied by adhesion tissues. Blue indicates DAPI staining of nuclei. Scale bar, 200 μm. Investigation (e) and quantitative analysis (f) of ROM at 14 days, 21 days, and 28 days. Quantitative analysis of gliding resistance (g), maximum tensile force (h), and stiffness (i) of repaired tendons. * indicates P < 0.05. B, bone; D, day; M, muscle; R, repaired site; ROM, range of motion; T, tendon

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