Fig. 6 | Bone Research

Fig. 6

From: Fragile X Messenger Ribonucleoprotein 1 (FMR1), a novel inhibitor of osteoblast/osteocyte differentiation, regulates bone formation, mass, and strength in young and aged male and female mice

Fig. 6

Deletion of FMR1 in IDG-SW3 osteocytic cells leads to increased mineralization and expression of osteocytic genes. a FMR1 gene expression level was assessed by qPCR in IDG-SW3 scrambled control (scr) and FMR1 knockout (FMR1-) cells (n = 3 per group). b Mineralization was induced in IDG-SW3 in the presence of osteoblastogenic media for 28 days (n = 8 per group) and was assessed by Alizarin red staining. c Gene expression in IDG-SW3 scr and FMR1- cells at their proliferating and differentiated stage (n = 3 per group). Images are representative of mineralized cultures after 28 days. Scale bar corresponds to 50 µm. Box boundaries indicate 25th to 75th percentile, and the horizontal lines corresponds to the median. Vertical lines correspond to minimum to maximum values, and each dot corresponds to an individual sample. *P < 0.05 vs. FMR1y/+ mice for males by student’s t-test and vs. FMR1+/+ by one way ANOVA for females. Detailed statistical analyses are included in Table S3

Back to article page