Fig. 7
FMR1 knock down in MLO-Y4 osteocytic cells and knockout in osteocytes isolated from FMR1-deficient mice results in increased osteocyte dendricity. a FMR1 gene expression level was assessed by qPCR in MLO-Y4 scramble (scr) and FMR1- cells (n = 3 per group). Representative images show effective green fluorescent protein expression following silencing. Scale bar corresponds to 25 μm. b Number of cells with 5 or more dendrites in MLO-Y4 scr and FMR1- cells (n = 3 per group). Representative images stained with H&E are shown. Scale bar corresponds to 40 μm. c Distribution of dendrite number in cells isolated from wild type and FMR1-deficient male and female mice. Representative images of cells stained with H&E are shown. Scale bar corresponds to 40 µm. d Mean dendrite number/cell and percentage of cells with 5 or more dendrites in primary osteocytes isolated from wild type and FMR1-deficient mice. Box boundaries indicate 25th to 75th percentile, and the horizontal lines corresponds to the median. Vertical lines correspond to minimum to maximum values, and each dot corresponds to an individual sample. *P < 0.05 vs. FMR1y/+ mice for males by student’s t-test and vs. FMR1+/+ by one way ANOVA for females. Detailed statistical analyses are included in Table S3 (in vitro) and S4 (ex vivo)
