Fig. 3

Depletion of CUL4B in MSCs exacerbated age-related bone loss and MAT accumulation. a Representative micro-CT three-dimensional reconstruction images of trabecular bone of femurs from 6-, 12- and 18-month-old WT and MKO mice. Scale bar, 0.3 mm. b–f Quantitative measurements of the trabecular bone mineral density (BMD) (b), bone volume/total volume (BV/TV) (c), trabecular number (Tb.N) (d), trabecular thickness (Tb.Th) (e) and trabecular separation (Tb.Sp) (f) of femurs from 6- (n = 5;5), 12- (n = 5;4) and 18-month-old (n = 5;7) WT and MKO mice by micro-CT. g Representative H&E staining of 6-, 12- and 18-month-old mouse femurs. Scale bar, 200 μm. h Representative IHC images of OCN, osteoblast number (Ob.N/BS) and osteoblast surface (Ob.S/BS) in 12-month-old WT and MKO mice (n = 7). Scale bar, 40 μm. i ELISAs of serum OCN in 6- (n = 6), 12- (n = 6) and 18-month-old (n = 6) WT and MKO mice. j Adipocyte number per tissue area and area of adipocytes per tissue area measured based on H&E images of 2- (n = 6) and 18-month-old (n = 6) WT and MKO mouse femurs. k Representative IHC of FABP4 and IOD quantification of 2- (n = 4) and 18-month-old (n = 4) WT and MKO mice. Scale bar, 40 μm. Error bars represent standard errors. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.000 1; ns, no significance